解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The ai...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903322216671
更新日期:2003-05-01 00:00:00
abstract::We have determined the expression pattern of all 11 human replication-dependent histone H3 genes in three fetal human tissues (bladder, liver, and lung) and in eight human cell lines by RNase protection assay. In the fetal human tissues, all 11 genes were expressed to a varied extent. However, the relative contributio...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903321908629
更新日期:2003-04-01 00:00:00
abstract::A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG) has been known to possess antiproliferative properties. In this study, we investigated the anticancer effects of EGCG in human papillomavirus (HPV)-16 associated cervical cancer cell line, CaSki cells. The growth inhibitory mechanism(s) and regulation o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454903321655846
更新日期:2003-03-01 00:00:00
abstract::Herpes simplex virus (HSV) is a neurotropic DNA virus with many favorable properties as a gene delivery vector. HSV is highly infectious, so HSV vectors are efficient vehicles for the delivery of exogenous genetic material to cells. Viral replication is readily disrupted by null mutations in immediate early genes that...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902762053864
更新日期:2002-12-01 00:00:00
abstract::The human ADH1A, ADH1B, and ADH1C genes encode alcohol dehydrogenases (ADHs) that metabolize ethanol. They evolved by recent tandem duplications and have similar proximal cis-acting elements, but differ in tissue-specificity. We hypothesized that distal cis-acting elements confer tissue-specificity. In this article, w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320908441
更新日期:2002-11-01 00:00:00
abstract::Chromosomal gain on 1q23-24 is a cytogenetic finding found in approximately 30% of bladder tumors. Currently, no defined or candidate tumor-associated genes from this region have been identified. The objective of this study was to identify and quantitate the expression of putative cancer genes located at this chromoso...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902760599681
更新日期:2002-10-01 00:00:00
abstract::The HIV-1 regulatory proteins Tat and Rev and the accessory proteins Vpr, Vpu, and Vif are essential for viral replication, and their cytoplasmic production suggests that they should be processed for recognition by cytotoxic T lymphocytes. However, only limited data is available evaluating to which extent these protei...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902760330219
更新日期:2002-09-01 00:00:00
abstract::Oxysterols are oxygenated derivatives of cholesterol. They have been shown to influence a variety of biological functions including sterol metabolism, lipid trafficking, and apoptosis. Recently, 12 human OSBP-related genes have been identified. In this study, we have identified a family of 12 oxysterol-binding protein...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902320308942
更新日期:2002-08-01 00:00:00
abstract::The aim of this article is to review our current understanding of the role of cytokines, chemokines, T cells, Langerhans cells, and neutrophils (PMN) and their interactions in vivo in the host response to Pseudomonas aeruginosa ocular challenge. The cellular/cytokine network in vivo has begun to be unraveled, and the ...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/10445490260099665
更新日期:2002-05-01 00:00:00
abstract::Transgenic mice expressing interleukin-12 (IL-12) under the glial fibrillary acidic protein (GFAP) promoter were evaluated for their sensitivity to herpes simplex virus type 1 (HSV-1) infection of the cornea. There was a modest but significant decrease in the infiltration of mononuclear cells in the cornea of the GFAP...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490260099764
更新日期:2002-05-01 00:00:00
abstract::Heme plays a significant pathogenic role in several diseases involving the kidney. The cellular content of heme, derived either from the delivery of filtered heme proteins such as hemoglobin and myoglobin, or from the breakdown of ubiquitous intracellular heme proteins, is regulated via the heme oxygenase enzyme syste...
journal_title:DNA and cell biology
pub_type: 杂志文章,评审
doi:10.1089/104454902753759726
更新日期:2002-04-01 00:00:00
abstract::Transfection of keratinocytes with plasmid DNA leads to the loss of detectable DNA-binding activity of CCAAT displacement protein but not of Yin Yang 1, as monitored by electrophoretic mobility shift assay. This phenomenon was found to be attributable to the presence of plasmid DNA in the nuclear extracts prepared fro...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454902753604989
更新日期:2002-02-01 00:00:00
abstract::Type 2 cystatins comprise a class of cysteine peptidase inhibitor presumed to mediate protective functions at various locations, including the oral cavity. Seven cystatin genes are clustered within a 300-kb region of human 20p11.2. "Salivary" cystatins, encoded by CST1, 2, 4, and 5, are present in saliva at significan...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490252810311
更新日期:2002-01-01 00:00:00
abstract::Most of the signal pathways involved in ultraviolet (UV)-induced skin carcinogenesis are thought to originate at plasma membrane receptors. However, UVA-induced signal transduction to downstream ribosomal protein S6 kinases, p70(S6K) and p90(RSK), is not well understood. In this report, we show that UVA stimulation of...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753438589
更新日期:2001-12-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder characterized by overproduction of beta-amyloid (Abeta), which is formed from amyloid precursor protein (APP), with the subsequent pathologic deposition of Abeta in regions of the brain important for memory and cognition. Recently, vaccination of murine models o...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490152717587
更新日期:2001-11-01 00:00:00
abstract::Cytochrome P450c17 catalyzes the 17alpha-hydroxylase/17,20 lyase activity needed for sex steroid synthesis. We recently characterized the nuclear phosphoprotein SET as a novel transcriptional regulator that binds to the -447/-399 region of the rat P450c17 gene, along with the transcription factors COUP-TF II, NGF-IB, ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901753340604
更新日期:2001-10-01 00:00:00
abstract::We cloned and sequenced a 1378 bp BamHI satellite DNA fraction from the water buffalo Bubalus bubalis and have studied its expression in different tissues. The GC-rich sequences of the resultant contig pDS5 crosshybridize only with bovid DNA and are not conserved evolutionarily. Typing of buffalo genomic DNA using pDS...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901317095007
更新日期:2001-09-01 00:00:00
abstract::Polyhomeotic is a member of the Polycomb group of genes. The products of this group are chromatin-associated proteins that act together as multimeric complexes. These proteins are required for the maintenance of target gene repression in a permanent and heritable manner during development. In order to better understan...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901316976118
更新日期:2001-08-01 00:00:00
abstract::Our previous study showed that the mutation hotspots of the K-ras proto-oncogene in human functional adrenocortical tumors are in codons 15, 16, 18, and 31, thus differing from the sites in other tumors. In addition, analyzing the K-Ras protein by a recombinant DNA technique showed that the activity of endogenic GTPas...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901750219116
更新日期:2001-04-01 00:00:00
abstract::Type 1 diabetes results in most cases from the destruction of insulin-secreting beta cells by the immune system. Several immunization methods based on administration of autoantigenic polypeptides such as insulin and glutamic acid decarboxylase (GAD) have been used to prevent autoimmune diabetes in the non-obese diabet...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454901300069022
更新日期:2001-03-01 00:00:00
abstract::Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490150504666
更新日期:2001-01-01 00:00:00
abstract::The glandular kallikrein family is composed of structurally related serine proteases. Studies show that the mouse family encompasses at least 14 highly conserved functional genes, but of these only the tissue kallikarein has a human ortholog. In man, the tissue kallikrein display high sequence similarity with prostate...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900750058080
更新日期:2000-12-01 00:00:00
abstract::Six identical cDNA clones corresponding to an RNA of 1685 nucleotides that is enriched in mouse sperm compared with testis were isolated from a mouse testis cDNA library. The sequence of these clones corresponds to the 16S mitochondrial RNA plus an inverted repeat of 120 bp covalently joined to the 5' end of the RNA. ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900439809
更新日期:2000-09-01 00:00:00
abstract::Streptococcus mutans plays a primary role in the formation of dental caries. Previously, in our laboratory, an S. mutans genomic library was prepared, and the wapA gene was cloned into the shuttle vector, pSA4/4B2. To generate overexpression of wapA and to facilitate efficient purification of the WapA protein for use ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/10445490050085898
更新日期:2000-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) is known for its ability to infect immune cells, including T-cells and macrophages. The 96-amino acid Vpr, a virion-associated protein, is essential for viral replication in monocytes/macrophages and increases viral replication in primary and established T-cell lines. The Vp...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900314564
更新日期:2000-03-01 00:00:00
abstract::The CAT gene is widely used as a reporter in eukaryotic systems because of the efficient translation of its mRNA. We report here that a sequence occurring in the CAT mRNA at +15 nucleotides from CAT AUG is essential for translation. This sequence includes a stem-loop structure, which, however, exhibits a calculated st...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454900314690
更新日期:2000-01-01 00:00:00
abstract::Porifera (sponges) constitute the lowest metazoan phylum. Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314746
更新日期:1999-12-01 00:00:00
abstract::The prototypic member of the transforming growth factor beta family is TGFbeta1, which is known to be important in extracellular matrix production, cell proliferation, and cell differentiation. Specifically in the pituitary lactotroph, TGFbeta1 inhibits prolactin (PRL) peptide secretion, PRL mRNA levels, and PRL gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314863
更新日期:1999-11-01 00:00:00
abstract::The mdmx gene was shown to possess high homology to the mdm-2 gene and to encode a protein that can bind p53 and block p53 transactivation. Because Mdm-2 protein blocks the growth-suppressive activity of the p53 tumor-suppressor protein through similar activities, we examined the expression patterns of mdmx to determi...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999314971
更新日期:1999-09-01 00:00:00
abstract::Tumor cells express lower levels of manganese superoxide dismutase (MnSOD) than their normal counterparts. Enforced expression of MnSOD reverses the malignant phenotype of many transformed cells, suggesting that SOD2 is a tumor suppressor. The SOD2 gene contains a large CpG island spanning > 3.5 kb that starts near th...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315051
更新日期:1999-08-01 00:00:00
abstract::The distribution and evolutionary pattern of the conserved microsatellite repeat sequences (CA)n, (TGG)6, and (GGAT)4 were studied to determine the divergence time and phylogenetic position of the water buffalo, Bubalus bubalis. The mean allelic frequencies of these repeat loci showed a high level of heterozygosity am...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315231
更新日期:1999-06-01 00:00:00
abstract::Nitric oxide synthase (NOS) is the enzyme responsible for the conversion of L-arginine to L-citrulline and nitric oxide. Dimerization of the enzyme is an absolute requirement for catalytic activity. Each NOS monomer contains an N-terminal heme-binding domain and a C-terminal reductase domain. It is unclear how the red...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315286
更新日期:1999-05-01 00:00:00
abstract::Liver-expressed chemokine (LEC) is a CC chemokine that is selectively expressed in the liver. We report here the structures of the human and mouse genes for LEC. The human LEC gene (SCYA16) was isolated from a bacterial artificial chromosome (BAC) clone that also contained CC chemokine genes for MPIF-1/Ckbeta8, HCC-2/...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315330
更新日期:1999-04-01 00:00:00
abstract::As a preliminary step toward muscle-mediated gene therapy in the mucopolysaccharidosis (MPS) type VI cat, we have analyzed the transcriptional regulation of feline N-acetylgalactosamine 4-sulfatase (f4S) gene expression from various retroviral constructs in primary cultures of muscle cells. Two retroviral constructs w...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315402
更新日期:1999-03-01 00:00:00
abstract::Promoter elements located up to 2 kb upstream of the apolipoprotein A-I (apoA-I) gene are necessary for apoA-I expression in liver and intestine cells in tissue culture. In transgenic mice, a distal enhancer located between the apoA-IV and apoC-III genes is additionally necessary for tissue-specific expression of apoA...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315493
更新日期:1999-02-01 00:00:00
abstract::Minimally modified low-density lipoprotein (MM-LDL) is regarded as a major risk factor for the development of atherosclerosis. In this report, we show that this lipoprotein complex can induce expression of an inflammatory protein, serum amyloid A (SAA), in monocyte/macrophage cells, a key cell type implicated in the p...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/104454999315637
更新日期:1999-01-01 00:00:00
abstract::Homomeric and heteromeric interactions among cell-surface tyrosine kinase receptors belonging to the ErbB family lead to intracellular signaling cascades which are involved in cell activation, cytoskeletal interactions, and cellular transformation leading to neoplasia. Monoclonal antibodies which specifically bind to ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.1031
更新日期:1998-12-01 00:00:00
abstract::At supramolecular resolution, DNA synthesis begins at preferred replication origins in the chromosomes of metazoan cells. To characterize one of these origins in detail, the initiation of replication was examined in the HeLa c-myc origin. Polymerase chain reaction (PCR) amplification of size-fractionated nascent chrom...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.885
更新日期:1998-10-01 00:00:00
abstract::The aromatic hydrocarbon receptor (AHR) is a ligand-activated transcription factor that regulates the expression of several drug-metabolizing enzymes and has been implicated in immunosuppression, teratogenesis, cell-specific hyperplasia, and certain types of malignancies and toxicities. The mouse Ahr gene 5' proximal ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.811
更新日期:1998-09-01 00:00:00
abstract::Screening of a mouse kidney cDNA library with a HNF-3/fork head domain probe revealed cDNA Hfh-1L containing the highly conserved fork head DNA-binding domain. The Hfh1L cDNA shows 92.7% homology at the nucleic acid level with the fork head gene HFH-1 from rat. Southern blot analyses demonstrated that the Hfh-1L gene ...
journal_title:DNA and cell biology
pub_type: 杂志文章
doi:10.1089/dna.1998.17.679
更新日期:1998-08-01 00:00:00